Plasmid

Part:BBa_K1453304:Design

Designed by: Binhan Hao   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-16)


pBluescript II KS(+)_5_copy


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 744
    Illegal PstI site found at 738
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 744
    Illegal PstI site found at 738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 744
    Illegal BamHI site found at 726
    Illegal XhoI site found at 777
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 744
    Illegal PstI site found at 738
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 744
    Illegal PstI site found at 738
    Illegal NgoMIV site found at 328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2150
    Illegal SapI site found at 1067


Design Notes

pBluescript II KS(+)


Source

pBluescript II KS(+)

References